ANALYTICAL METHOD VALIDATION OF CYCLAMATE IN RED SYRUP IN NORTH BANJARMASIN USING UV-VIS SPECTROPHOTOMETER

Abstract

Cyclamate is an artificial sweetener that is not allowed for public consumption, because it is
specifically permitted for diabetics and consumers with low-calorie diets. Syrup usually
added as a flavor enhancer (sweetener), color, and aroma. The purpose of adding artificial
sweeteners to syrup is to reduce production costs because cyclamates have a higher
sweetness and lower price than sugar. The purpose of this study was to determine the content
and levels of cyclamate in syrup sold in various stalls and street sellers in the North
Banjarmasin area, and to determine the data validation method with linearity, precision,
accuracy, LOD, and LOQ. The results of the qualitative test showed that 4 of the 9 positive
samples contained cyclamate (samples A, C, E, and I). The results of the method validation
parameter test for linearity at 20, 40, 60, 80, 100, and 120 ppm gave a value of the
correlation coefficient (r ) of 0.9987 with an LOD value of 6.4307 ppm, LOQ of 21.4359
ppm,d precision value of 0.74%, and an accuracy value of 89.5126%. The results of the
quantitative tests were carried out on four samples, namely samples A, C, E, and I, with
cyclamate levels of 12.9423, 31.9833, 23.4166, and 115.6469 mg/kg, respectively.

Keywords: Cyclamate, Syrup, Precipitation, Validation Method, UV-Vis Spectrophotometry