PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK METANOL KULIT JERUK SAMBAL SECARA SPEKTROFOTOMETRI UV-VISIBEL
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Abstract
Jeruk Sambal (Citrus microcarpa Bunge) seringnya dimanfaatkan sebagai tanaman obat tradisional di Kalimantan Barat, seperti antioksidan dan antibakteri dikarenakan adanya senyawa flavonoid dan polifenol. Penelitian ini bertujuan untuk menentukan kadar flavonoid total dari ekstrak metanol kulit jeruk sambal. Penentuan kadar flavonoid total menggunakan metode spektrofotometri UV-Visibel dengan kuersetin sebagai pembanding. Pengukuran panjang gelombang maksimum kuersetin dilakukan pada panjang gelombang 380 nm – 780 nm dan diperoleh panjang gelombang maksimum kuersetin pada 432 nm. Kadar flavonoid total dihitung dengan persamaan regresi linear y = 0,0068 x + 0,1132 dengan koefisien relasi R2 = 0.9712 yang diperoleh dari kurva kalibrasi kuersetin. Hasil skrining fitokimia menunjukkan kulit jeruk sambal mengandung flavonoid dengan kadar flavonoid total sebesar 0,3324 mg/g.
Kata kunci:Â Kulit Jeruk Sambal, Penetapan Kadar, Flavonoid, Spektrofotometri UV-Visibel
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